Performance of Aptima HPV E6/E7 mRNA Test for Detection of Cervical Lesions in a Large Chinese Population

Background: we aimed to examine the effectiveness of aptima hpv e6/e7 mrna test for detection of cer-vical lesions in a large chinese population.methods: overall, 4,350 women, who received simultaneously aptima hpv e6/e7 mrna test and hpv dna test, followed by cervical biopsy in the department of gynecology of the second affiliated hospital of soochow university, jiangsu province, china from 2016-2020, were recruited. the detection of cervical lesions was compared between aptima hpv e6/e7 mrna test and hpv dna test.results: overall, hpv dna test exhibited a higher detection of all cervical lesions than aptima hpv e6/e7 mrna test (p<0.05), and showed a higher efficacy for detection of normal tissues and chronic cervicitis (p<0.05) and low-grade squamous intraepithelial lesions (lsils) (p<0.05) than aptima hpv e6/e7 mrna test; while aptima hpv e6/e7 mrna test showed a greater detection of high-grade squamous intraepithelial lesions (hsils) (p<0.05) and invasive cervical carcinoma than hpv dna test. aptima hpv e6/e7 mrna test exhibited a higher specificity p<0.05), positive and negative prediction rates than hpv dna test for detec-tion of cervical lesions, and the sensitivity was comparable between the two tests (p>0.05).conclusion: aptima hpv e6/e7 mrna test gradually improves the detection of cervical lesions with disease severity, and shows a higher specificity, positive and negative prediction rates and comparable sensitivity for detection of clinical cervical lesions as compared with hpv dna test.