Association of CCL5 rs2107538, and CCL2 rs3760396 Gene Polymorphisms with the Risk of Cardiovascular Disease

Background: chemokines are proinflammatory cytokines that play key roles in development of cardiovascular diseases (cvd). chemokine-induced recruitment of peripheral leucocytes to tissues is a crucial step in the cvd progression. cc chemokines ligand 5, 2 (ccl5 and ccl2), have been characterized as emerging inflammatory biomarkers of atherosclerotic cvd. the aim of this study was to find out whether genetic polymorphisms of ccl5 -403 g>a (rs2107538) and ccl2 –927 g>c, (rs3760396) were associated with the risk of cvd. methods: in this case-control study, 500 iranian individuals including 250 cvd patients and 250 healthy subjects as the control group participated in 2017. genotyping of ccl5 -403 g>a and ccl2 –927 g>c polymorphisms were executed using tetra-arms pcr method. results: at genotypic level both ccl5 -403 g>a and ccl2 –927 g>c polymorphisms were not associated with the risk of cvd (p>0.05), even after adjustment by age, sex, race, and history of hypertension, dm and smoking. however, the ccl2 –927 c allele was associated with an increased risk of cvd (or=1.42, p=0.050) with a higher prevalence in cvd patient than in controls (17% vs. 12%). moreover, the haplotype analysis revealed that ccl5/ccl2 haplotype (g/c) was a risk factor for cvd (or=2.13, p=0.001), and that carriers of this haplotype were at 2.13-fold higher risk of cvd than subjects with g/g haplotype. conclusion: ccl2 –927 c variant and ccl5/ccl2 haplotype (g/c) were associated with susceptibility to cvd, and were risk factors for cvd in our population but more studies with large sample size are recommended.