Pyrrolidinedithiocarbamic Acid Ammonium Salt Inhibits Apoptosis and Phenotypic Transformation of Co-Culture of Myeloma Cells and Renal Tubular Epithelial Cells by Reducing the Secretion of Light Chain Protein

Background: we investigate the effects of nfκb inhibitor pyrrolidinedithiocarbamic acid ammonium salt (pdtc) on the viability, apoptosis and cell phenotype of hk-2 cells in the co-culture system of myeloma cells in renal tubular epithelial cells. methods: this study was performed in qiqihar medical university, qiqihar, china from jun 2018 to jan 2019. rpmi-8226 cells and hk-2 cells were inoculated in the co-culture chamber and cultured to establish the co-culture system. an immunoturbidimetric assay was performed to detect κ light chain and λ light chain in rpmi-8226 cells. the effect of pdtc on the secretion of κ light chain and λ light chain of rpmi-8226 cells was detected by immunoturbidimetry and the ratio was calculated. results: pdtc significantly increased the viability of hk-2 cells. pdtc reduced the apoptosis of renal tubular epithelial cells. after pdtc treatment, the expression of cell surface marker e-cadherin decreased, and the expression of α-sma increased, which induced the renal interstitial fibrosis. the secretion of κ light chain and λ light chain of rpmi-8226 cells was significantly decreased after the addition of pdtc, but the ratio was not changed. conclusion: pdtc can inhibit the cell activity, promote apoptosis, and reduce the secretion of secretion of κ light chain and λ light chain through inhibiting the nf-κb pathway activation of myeloma cell rpmi-8226.