antioxidative and antiinflammatory activity of kenya grade aa green coffee bean extracts

Background: kenya aa green coffee bean extracts were tested for natural ingredients used for antioxidative and antiinflammatory purposes in cosmetic products.methods: antioxidative activities were measured by total polyphenol, 1,1diphenyl2picrylhydrazyl (dpph), and the 2,2′azinobis (3ethylbenzothiazoline6sulfonic acid) (abts) assays. antiinflammatory activities were evaluated via nitric oxide (no) assays, and through quantification of inducible nitric oxide synthase (inos), and cyclooxygenase2 (cox2) protein expression by western blotting. data analyses were performed using independent student’s ttests, with statistical significance set at p < 0.05..results: total polyphenol content of water and ethanol extract was 169.0 ± 3.1 mg and 300.34 ± 16.6 mg tannic acid/g dry weight, respectively. the dpph and abts radical scavenging activities of all the extracts were significantly increased in a concentrationdependent manner. kenya aa green coffee bean extracts were toxic at a concentration of 1,000 µg/ml in raw 264.7 cells. antiinflammatory activity as determined by no assay showed that lipopolysaccharide (lps)induced no was significantly inhibited following treatment with kenya aa green coffee bean extracts in a concentrationdependent manner. inos and cox2 protein expression was also significantly inhibited following treatment.conclusion: these results highlight the potential of kenya aa green coffee bean extracts as a naturally active antiinflammatory agent in cosmetic products.