taurine activates bmp2/wnt3amediated osteoblast differentiation and mineralization via akt and mapk signaling

Background: we aimed to elucidate the preventive effects of taurine against osteopenia in ovariectomized (ovx) rats and the mechanisms by which taurine regulates osteoblastogenesis in vitro and in vivo.methods: the effects of the taurine on human osteoblast mg63 cell differentiation and osteoblastogenesis effect in ovx rat were examined konkuk university in 2018 by evaluating osteoblast differentiation, and expression of osteoblastspecific factors by western blotting analysis.results: taurine supplementation significantly improved alkaline phosphatase (alp) activity and mineralization in a concentrationdependent manner. further, taurine induced the expression of osteogenic growth factors such as bone morphogenetic protein2 (bmp2), runtrelated transcription factor 2 (runx2), small mothers against decapentaplegic 1/5/8 (smad1/5/8), winglesstype mmtv integration site family member 3a (wnt3a), and collagen type 1 (col1) via mitogenactivated protein kinase (mapk) and serine/threonine protein kinase (akt). moreover, the runx2 activity of the taurinetreated group was enhanced by proteinprotein interactions such as wnt3ainduced pakt/runx2 and bmpmediated smads/mapk/runx2 interactions.conclusion: our in vitro and in vivo results suggested that taurine can be considered as a potential therapeutic candidate agent for preventing bone loss in postmenopausal osteoporosis.