evaluation of (gtg) 5-pcr for genotyping of klebsiella pneumonia strains isolated from patients with urinary tract infections

Background: klebsiella pneumonia is one of the common causes of pneumonia and bacteremia in intensive care patients. the present study was aimed to determine the capability of (gtg) 5-pcr assay for molecular typing of k. pneumonia strains isolated from patients with urinary tract infections. methods: in this descriptive-sectional study, k. pneumoniae strains were collected from hospitalized patients with urinary tract infection in baqiyatallah hospital, tehran, iran during 2017-2018. isolates were identified by con-ventional microbiological tests. bacterial dna was extracted using boiling method and (gtg) 5-pcr assay was used for subtyping of the isolates. for clustering of isolates, dendrogram was generated according to the un-weighted pair group method with arithmetic (upgma). results: overall, 88 k. pneumoniae isolates were isolated and subjected to the molecular typing study. the (gtg) 5–pcr assay was able to differentiate the k. pneumoniae strains into 9 clusters including g1-g9. genotype clusters g4 and g9 consist of highest (26) and lowest (1) number isolate, respectively. conclusion: the k. pneumonia strains isolated under the study belonged to various clones and the (gtg) 5-pcr assay as simple and rapid method can be a powerful tool for molecular typing of k. pneumoniae strains.