SSU- rRNA Gene Analysis of Cryptosporidium spp. in HIV Positive and Negative Patients

Cryptosporidium is an apicomplexan parasite of humans and a wild range of domestic as well as wild animals. an 833-bpfragment of the 18s-rrna gene was used to identify cryptosporidium spp. recovered from children and adult patients, inhuman immunodeficiency virus (hiv) positive and negative patients in iran. initial identification of cryptosporidiosis was carried out by ziehl-neelsen acid-fast staining method of stool samples. the samples, then, were identified specifically bynested pcr, targeting the most polymorphic region of the 18s-rrna gene. the genotype encountered was detected by restrictionendonuclease digestion of the nested-pcr product. among 17 analyzed isolates, two different genotypes ofcryptosporidium were identified; 24% of the isolates belonged to c. parvum human genotype, and 76% to the potentiallyzoonotic species of c. parvum bovine genotype. the results of the present study showed that in contrast to hiv negative individuals, hiv positive individuals were more likely to be infected with zoonotic genotypes of the parasite; it was also confirmed the fact that zoonotic transmission of the parasite in iran was as frequent as the transmission of anthroponotic origin. these outcomes are helpful for researchers to establish the corresponding prevention and treatment measures.