Prevalence of Echinococcus spp. Infection Using Coproantigen ELISA among Canids of Moghan Plain, Iran

Background: echinococcosis is one of the most important helminthic zoonotic diseases in iran. intestinal scraping technique(ist), the traditional method for diagnosis of the infection in definitive hosts, has many disadvantages including lowsusceptibility and being expensive, hazardous and laborious. detection of coproantigens in fecal samples by enzyme-linkedimmunosorbent assay (ca-elisa) is known as a useful tool for intravital mass-screening of definitive host populations.this study was performed to determine the prevalence of echinococcus spp. infection among canids in moghan plain, theonly area in iran known to be endemic for e. multilocularis.methods: one hundred thirty eight fecal samples were collected from red foxes and domestic dogs in three counties ofmoghan plain namely pars abad, bileh savar and germi. the samples were examined using an elisa, designed for thedetection of echinococcus-specific coproantigen and the formalin-ether concentration method as well.results: totally, out of 138 fecal samples, 27 (21.6%) turned positive for echinococcus. coproantigen was detected in16.7% and 27.1% of red foxes and domestic dogs, respectively. formalin-ether concentration method revealed that 43(31.2%) of samples harbored at least one parasitic helminth, but taenia eggs were detected only in 3 fecal samples. sincecoproantigen presence reflects current intestinal infection with adult worms, ca-elisa can be regarded as one of the mostuseful immunological tools for diagnosis of echinococcus infection. besides, the high susceptibility, low cost and rapiditymake it the method of choice for epidemiological surveys in endemic areas of iran.