Genotyping Pattern Among Iranian Hcv Positive Patients

Background: successful treatment to eliminate hcv rna depends on the identified genotype. in the present study, wecompared the frequency of different hcv genotypes, during four years study (2004 till 2008).methods: sera specimens were received from 16 provinces of iran. we used high pure viral nucleic acid purification kitfor extraction and samples were tested with improved form of rt-pcr technique. hcv genotypes were determined usingamplisense pcr kit and amplicor hcv monitoring version 2 test utilized a reverse transcription (rt)-pcr approach toquantitative hcv rna. two hundreds six hcv positive specimens were entered to the study out of 389 tested samples.results: type 3a was the most frequent type (46.6%), followed by type 1 (including 1a and 1b with 25.73% and 17.47% foreach respectively) with 43.2%. looking through collected results of the four years study confirmed the rate of hcv infectionin those single genotypes 1b, 3a were slightly increased from 12.22% and 38.88% in the first year to 18.66 and 46.51%in the fourth year of the study period.conclusion: the analyzed data proved that some patients were infected with two different types. high viral load was alsomore correlated to genotype 1 than other types.