Microtitration of Rubella Virus in Monovalent Vaccinal Products

Background: potency test for control of rubella vaccine is a significant factor to qualify production line and vaccination pro­gram. for this reason, who recommends to use the microtitration method by both vaccine companies and control laborato­ries. then the study was done to improve this test.methods: three rubella virus samples, including an in-house standard, a lot of vaccine and an in- process product, were tit­tered in cell culture tubes. then micro titration steps were tested on 96-well microplate using cocultivation of standard ru­bella vaccine dilutions and rk- 13 cell line. after 6-7 days, final reading was done and calculated the titer. two other sam­ples were assayed with the micromethod.results: titer reduction less than 0.5 log was acquired for each sample during frequent tests and between two methods.conclusion: the procedure was profitable and accurate for potency and identity tests of rubella virus vaccine, on the basis of who recommendations.