Mechanism of Probiotic Vsl#3 Inhibiting Nf-Κb and Tnf-Α on Colitis Through Tlr4-Nf-Κb Signal Pathway

Background: we aimed to investigate the effect of probiotic vsl#3 on nf-κb and tnf-α in rats with colitis and the correlation with tlr4-nf-κb signal pathway. methods: sixty sprague dawley (sd) rats were divided into the control, model and therapy groups (n=20) according to the random number table. rats in the model and therapy groups were modeled for colitis, and rats in the therapy group were intragastrically administered with probiotic vsl#3. the expression of tlr4 and nf-κb protein, and the levels of nf-κb, tlr4, and tnf-α mrna in the colon tissue were detected. the concentration of tnf-α in the serum after modeling but before intragastric administration (t0), 3d (t1) and 7d after intragastric administration (t2) was detected. results: the expression of tlr4 and nf-κb p65 protein, and the levels of tlr4, nf-κb, and tnf-α mran in the therapy group decreased (p < 0.001). at t0, t1, and t2, the concentration of tnf-α in the model and control groups increased (p < 0.001). tlr4 and nf-κb in the therapy group were positively correlated with tnf-α mran (p < 0.050).conclusion: in conclusion, probiotic vsl#3 inhibits the expression of nf-κb and tnf-α in rats with colitis through tlr4-nf-κb signal pathway, so it is expected to be a first choice drug for the treatment of colitis. conclusion: in conclusion, probiotic vsl#3 inhibits the expression of nf-κb and tnf-α in rats with colitis through tlr4-nf-κb signal pathway, so it is expected to be a first choice drug for the treatment of colitis.