Preparative SDS-PAGE Electroelution for Rapid Purification ofAlkyl Hydroperoxide Reductase from Helicobacter pylori

Background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. therefore,this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study wasto simplify the ahpc protein purification procedures.methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-ß-(-d glucopyranoside. the isolationand purification of ahpc protein were attempted by various techniques including ammonium sulfate precipitation, dialysis,preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and electroelution.results:a simple method was used for protein purification ahpc protein. one-dimensional preparative gel electrophoresis allows a single and short purification step; the high resolution capacity of this technique leads to a high level of purity of the protein. moreover, it avoids contamination by other non-specific proteins which often appear during protein purification bycolumn chromatography.conclusion: the present method is simple, rapid and makes it possible to preparate ahpc from h. pylori