Prevalence of Genes Encoding Bi-Component Leukocidinsamong Clinical Isolates of Methicillin–Resistant Staphylococcusaureus

Background: staphylococcus aureus has been recognized as a major human pathogen and is the major cause of nosocomialinfections. gamma-toxin, leukocidin and other bi-component toxins are a family of proteins encoded by the hlg and luk-pv,respectively. panton-valentine leukocidin (pvl) is an example of these toxins and causes leukocyte destruction and tissuenecrosis. the aim of this study was to determine the prevalence of bi-component leukocidin in methicillin – resistantstaphylococcus aureus (mrsa) isolates in staphylococcal infections.methods: collectively, 143 isolates of s. aureus were obtained from tehran university of medical sciences hospitals andconfirmed with biochemical tests. then polymerase chain reaction was used to detect luk-pv loci and luk-e/d. coagulasegene was used as internal control. the antibiotic susceptibility patterns of isolates were determined using disk diffusionmethod.results: out of 149 s. aureus isolates 24.2% were luk-pv positive and 73.8% were luk-e/d positive.conclusion: there was pvl–positive mrsa isolates with high prevalence in evaluated hospitals. the diseases from thesebacteria are with extensive necrosis, leucopenia and even death. we desire that, prevent from progress and death by diagnosisand right treatment.