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Enzymatic Hydrolysis of Used-Frying Oil Using Candida rugosa Lipase
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نویسنده
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Rashid Roslina ,Zaharudin Nor Athirah ,Idris Ani
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منبع
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jurnal teknologi - 2014 - دوره : 67 - شماره : 3 - صفحه:101 -107
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چکیده
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Hydrolysis of used-frying oil had been carried out by using an immobilized lipase from candida rugosa in solvent-free system. used-frying oil was considered as the substrate in this study due to abundance amount of used-frying oil present in malaysia as its disposal problem has become a very serious environmental issue. the high free fatty acids (ffa) content in used-frying oil has raised the interest for the utilization of this waste into valuable products. even though used-frying oil is not suitable for human consumption and being extensively used for the biodiesel production, ffa from used-frying oil could be utilized to produce various types of non-edible products. effects of enzyme loading, water content, reaction temperature, buffer ph and agitation speeds on the hydrolysis process were investigated. the experiments were conducted at constant reaction time of 3 hours. it was found that the effect of variables were very significant on the hydrolysis process. the hydrolysis process achieved the highest yield of fatty acids at enzyme concentration of 1.5% (w/v), buffer volume to oil volume ratio of 3:1, temperature of 40˚c, ph of 7, and agitation speed of 220 rpm. under these described conditions, it was found that nearly 97.15±1.31% of hydrolysis degree was achieved with 2533.33±26.67 μmol/ml of fatty acids was produced.
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کلیدواژه
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Used frying oil; Candida rugosa lipase; enzyme loading; hydrolysis; fatty acids
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آدرس
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Universiti Teknologi Malaysia, Faculty of Chemical Engineering, Department of Bioprocess Engineering, Malaysia, Universiti Teknologi Malaysia, Faculty of Chemical Engineering, Department of Bioprocess Engineering, Malaysia, Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering, Department of Bioprocess Engineering, Malaysia
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Authors
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