Optimization of Critical Medium Components for the Expression of Recombinant Human Transferrin in Insect Cells Baculovirus System

Human transferrin (htf) plays a big role in providing bacteriostatic functions as well as to transport iron from the storage part to all proliferating cells by receptor mediated endocytosis. insect cells baculovirus expression system has been widely used as an alternative expression system for the production of recombinant human transferrin (rhtf). this work focused mainly on the optimization of glutamine, glucose and lipid mixtures 1000x to increase rhtf yield. an experimental design involving 17 central composite design (ccd) experiments was employed and results were analyzed by statistica (statsoft v. 5.0). the response surface methodology (rsm) had identified the optimum values where glutamine=2211.20 mg/l, glucose=1291.95 mg/l, and lipid mixtures 1000x=0.64 %v/v. using the optimized parameters, the studies demonstrated an increase in the rhtf yield by three-fold from 19.89 μg/ml to 65.12 μg/ml