Reaction optimization of Aspergillus niger α-L-Arabinofuranosidase for improved arabinose production from kenaf stem

There are abundant of lignocellulosic biomass readily available with varying compositions. kenaf (hibiscus cannabinus) is one of this lignocellulosic biomass that has a high content of hemicellulose. this particular hemicellulose is composed of high arabinoxylan,which is a xylan backbone with arabinofuranosyl branches. in order to hydrolyze arabinoxylan,a branching enzyme is needed. therefore,α-l-arabinofuranosidase from aspergillus niger atcc120120 (anabfa) was used to hydrolyzed pre-treated kenaf and the reaction conditions were optimized using central composite design (ccd) to produce a significant amount of arabinose. there were 20 experiments conducted with 1.68 star points and 6 replicates at the centre points. the reaction conditions that were optimized are enzyme loading,substrate concentration and reaction time in which resulted with 88 u anabfa activity,0.9% (w/v) and 48 h,respectively. these optimized conditions managed to increase the yield of arabinose with 47.17 mg/g arabinose produced. © 2017 penerbit utm press. all rights reserved.