Methylation Status of Tumor-Related Genes in Mcf7, Mda-Mb-468 and Bt-474 Breast Cancer Cell Lines with Different Estrogen Receptor Status

Background:many breast cancer tumor suppressor genes have been reported toundergo hypermethylation, including er, rassf1a, hic, cdh1, gspt1, apc,twist and ccnd2. we first determined the promoter methylation of the above eighttumor suppressor genes as epigenetic markers of breast cancer in three cell lines withdifferent er status.methods: this study was an experimental study performed on three cell lines:er-positive mcf-7, er-negative mda-mb-468 and the er- and her2-positivebreast cancer cell line (bt-474). for this purpose dna was extracted from these celllines and gene promoter methylation was analyzed by methylation-specific pcr. results: our results showed that all studied genes were hypermethylated in er-negative mda-mb-468 cells. we detected hypermethylation of gspt1, rassf1aand ccnd2 genes in these three cell lines. in addition, the er-positive mcf-7 cellsshowed methylation of twist and apc genes.conclusion: the numbers of methylated molecular biomarkers is increased withtumor progression.this finding may be important in breast cancer therapy fordifferent er status.