n-nornuciferine promotes human non-small cell lung cancer a549 cells apoptosis via mir-361-3p/traf2/jnk axis

Background: n-nornuciferine (n-nf) is an aporphine alkaloid in lotus leaf with a good resource for obtaining the biologically active substances with antioxidant properties. the purpose of this study was to explore the effect of n-nf on cell viability and apoptosis in a549 cells in vitro culture and to explore its mechanism of action.method: in this experimental study, we cultured a549 cells in vitro and added different concentrations of n-nf for intervention to explore cell apoptosis and its mechanism. cell viability was detected by cck-8, and cell apoptosis level was detected by flow cytometry. the mechanism of action of n-nf was explored by regulating the expression of mir-361-3p. the data were analyzed using the one-way analysis of variance (anova) technique in spss 22.0 software, with statistical significance set at a level of p < 0.05.results: the study results showed that n-nf could significantly inhibit the viability of a549 cells and promote apoptosis. using dual-luciferase reporter gene and molecular biology detection experiments, it was found that n-nf inhibits the viability of a549 cells through inhibiting the expression of mir-361-3p and promoting the traf2/jnk pathway.conclusion: this study provides a basis for the development of n-nf as an anticancer drug and explains its mechanism of promoting apoptosis of a549 cells.