evaluation of the lncrna tmpo as-1 expression level in melanoma tissues in comparison to paired normal marginal tissues

Background: long noncoding rna (lncrna) is recognized as an essential controller of gene expression and other activities of the cells. additionally, lncrnas have a critical role in the progression and growth of human malignancies, like melanoma. among lncrnas, thymopoietin (tmpo)-antisense rna 1 (tmpo as- 1) has a significant role in melanoma. the current study aimed to determine the expression level of tmpo as-1 in melanoma patients.method: in this case-control research, 50 pairs of tumor and non-tumor tissues of melanoma patients were separated by the surgeon. subsequently, tmpo as-1 expression level in the tissues was evaluated. we used trizol to extract total rna from the tumor and non-tumor tissues, following which complementary dna was synthesized. the tmpo as-1 expression level of tmpo as-1 was evaluated via quantitative reverse transcription- polymerase chain reaction (qrt-pcr) technique. moreover, clinicopathological features of the melanoma patients were evaluated.results: our findings revealed that tmpo as-1 expression level was upregulated in the melanoma tissues in comparison with the non-tumor ones. remarkably, the tmpo as-1 expression level was considerably correlated to the clinicopathological characteristics of the patients, including lymph nodes and distant metastasis.conclusion: upregulation of tmpo as-1 in melanoma indicated that tmpo as- 1 and its downstream signal pathways could be used as a new target treatment option and prognostic tumor marker for melanoma.