umbelliprenin inhibited angiogenesis and metastasis of mda-mb-231 cell line through downregulation of cocl2 / egfmediated pi3k / akt / erk signaling

Background: breast cancer is known to be one of the most prevalent malignancies in women worldwide. umbelliprenin (umb) is a naturally-occurring component derived from plant species, which has shown anticancer properties. the present study aimed to evaluate the effect of umb on the pi3k / akt / erk signaling pathway and their products hif-1α / vegf in the mda-mb-231 cell line.method: in this experimental study, the cytotoxic effect of umb on mda-mb- 231 cells was evaluated using the mtt assay and the umb concentrations of ic5 and ic10 were selected for the signaling pathway study. mda-mb-231 cells were stimulated with egf and cocl2 and umb ic5 and ic10 effects on gene expression and translation was studied. pi3k / akt / mtor / s6k / erk1 and 2 / 4e-bp1 / hif-1α / hif-1α/ egfr / vegfr and vegf mrna expression, and vegf / hif-1α proteins were evaluated employing real time polymerase chain reaction and western blot analysis, respectively.results: the concentrations of umb in ic10 and ic5 were 20 and 10 μm, respectively. umb, specifically ic10, significantly inhibited pi3k, erk1, erk2, akt, mtor, hif1-α, hif1-β mrna, as well as hif-1α and vegf protein expression. conclusion: our results suggested that umb, a cytotoxic agent, inhibits pi3k / akt / erk signal pathway in the cocl2 or egf-stimulated mda-mb-231 cells.