Alteration of P53, hMLH1, and HER2 Genein Bangladeshi Gastric Cancer Patients:Their Association with H. pylori Infectionand Clinicopathological Factors

Background: gene alterations are required for the development of gastric cancer,which are influenced by environmental and host factors. we conducted the presentstudy to find the status of helicobacter pylori (h. pylori) infection and its associationwith altered genes p53, hmlh1, and her2 in gastric cancer patients and to analyzetheir correlation with clinical, pathological, and environmental factors.method: this was a cross-sectional study. for genetic (p53 and hmlh1) studyof the gastrectomized tissue dna extraction and optimization, we performed pcramplification and dna sequencing. her2 was studied by immunochemical technique.the results were matched with tumor status, age and sex, smoking, and h. pyloriantibody status of the patients to find their association.results: the mean age of the patients was 52.91 (±13.94) years. among the 45patients selected for genetic tests, 12 aged 40 or more and 33 aged over 40. amongthe genes, 33(73.3%) in p53 and 17(37.7%) in hmlh1 were mutated and 11(24.2%)in her2 were found to be overexpressed. chi square and regression analysis showedthat they all had associations with h. pylori positivity (p < 0.05, odds ratio >1).hmlh1 was associated with the location of the tumor, smoking, sex, blood group,and age, and p53 was found to be affected by extra salt intake, sex, blood group, andage of the patients (p ≤ 0.05 ).conclusion: genetic mutation was found in nearly all the patients with gastriccancer, which was significantly associated with h. pylori infection. mass eradicationof this organism might play a role in reducing cancer incidence in bangladesh.