Investigation of Programmed Cell Death-1 (PD-1) Gene Variations at Positions PD1.3 and PD1.5 in Iranian Patients with Non-small Cell Lung Cancer

Background: tumor cells express pd-1 ligands to bind pd-1 on immune cells and escape immune responses. in the present study, we aimed to investigate whether single nucleotide polymorphisms at positions pd1.3 (+7146, rs11568821) g/a, and pd1.5 (+7785 c/t, rs2227981) may be considered risk factors for susceptibility to nonsmall cell lung cancer in the iranian population. methods: this study enrolled 206 histopathologically confirmed lung cancer patients and 173 age/sex matched healthy controls. we performed pcr-rflp to determine the genotypes of the extracted genomic dna. results: the frequencies of pd1.3 gg, ga and aa genotypes were 171 (83%), 31 (15%) and 4 (1.9%) out of 206 patients, and 144 (83.2%), 26 (15%), and 3 (1.7%) out of 173 controls, respectively. the frequencies of pd1.5 cc, ct and tt genotypes were 78 (37.9%), 100 (48.5%), and 28 (13.6%) in patients, and 60 (34.7%), 89 (51.4%), and 24 (13.9%) in controls. there were no significant differences in genotype analysis between patients and controls at positions pd1.3 (p=0.98) or pd1.5 (p=0.80). no significant differences existed in the frequencies of alleles and haplotypes between the two groups (p>0.05). conclusion: our data have indicated no association between pd1.3 (+7146) g/a and pd1.5 (+7785) c/t with susceptibility to non-small cell lung cancer. investigation of other pd1 genetic variations and emerged haplotypes are required to completely define the role of pd1 genetic variations in susceptibility to lung cancer.