بررسی ریخت شناسی ریشه و برگ دو رقم عدس (lens culinaris medic) تحت تنش سایه و تلقیح با باکتری آزوسپیریلیوم

ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﻲ اﺛﺮ ﺗﻨﺶ ﻧﻮری و ﺑﻴﻮﭘﺮاﻳﻤﻴﻨﮓ در دو رﻗﻢ ﻋﺪس، آزﻣﺎﻳﺸﻲ زراﻋﻲ در ﻣﺰرﻋﻪ ﺗﺤﻘﻴﻘﺎﺗﻲ داﻧﺸﻜﺪه ﻛﺸﺎورزی داﻧﺸﮕﺎه اﻳﻼم در ﺳﺎل زراﻋﻲ 1391-92 ﺑﺼﻮرت ﻓﺎﻛﺘﻮرﻳﻞ درﻗﺎﻟﺐ ﻃﺮح ﭘﺎﻳﻪ ﺑﻠﻮکﻫﺎی ﻛﺎﻣﻞ ﺗﺼﺎدﻓﻲ ﺑﺎ 3 ﺗﻜﺮار اﺟﺮاﮔﺮدﻳﺪ. ﺗﻴﻤﺎرﻫﺎی آزﻣﺎﻳﺸﻲ ﺷﺎﻣﻞ ﺳﺎﻳﻪ اﻧﺪازی ﻋﺪم ﺳﺎﻳﻪ، 25، 50، 75 و 100 درﺻﺪ ﺳﺎﻳﻪ(، ﺑﻴﻮﭘﺮاﻳﻤﻴﻨﮓ ﺗﻠﻘﻴﺢ ﺑﺎ ﺑﺎﻛﺘﺮی آزوﺳﭙﻴﺮﻳﻠﻴﻮم ﺑﺮازﻳﻠﻨﺲ و ﻋﺪم ﺗﻠﻘﻴﺢ( و ارﻗﺎم ﻋﺪس زﻳﺒﺎ و ill4400( ﺑﻮد. ﻧﺘﺎﻳﺞ ﺣﺎﺻﻞ از اﻳﻦ ﭘﮋوﻫﺶ ﻧﺸﺎن داد ﻛﻪ ﺑﺎ اﻓﺰاﻳﺶ ﺳﻄﻮح ﺳﺎﻳﻪاﻧﺪازی وزن ﺗﺮ و ﺧﺸﻚ رﻳﺸﻪ، ﺣﺠﻢ رﻳﺸﻪ، ﻃﻮل رﻳﺸﻪ و ﻋﻤﻠﻜﺮد داﻧﻪ ﻛﺎﻫﺶ ﻣﻲﻳﺎﺑﺪ وﻟﻲ ﺳﺒﺐ اﻓﺰاﻳﺶ ﺷﺎﺧﺺ ﺳﻄﺢ ﺑﺮگ، ﻣﺤﺘﻮی ﻛﻞ آب ﺑﺮگ و ﺳﻄﺢ وﻳﮋه ﺑﺮگ ﻣﻲﮔﺮدﻳﺪ. در ﻫﺮ دو رﻗﻢ ﺗﻠﻘﻴﺢ ﺑﺎ ﺑﺎﻛﺘﺮی آزوﺳﭙﻴﺮﻳﻠﻴﻮم ﻣﻨﺠﺮ ﺑﻪ ﻛﺎﻫﺶ ﺧﺴﺎرت ﻧﺎﺷﻲ از ﺳﺎﻳﻪاﻧﺪازی و اﻓﺰاﻳﺶ رﺷﺪ و ﻋﻤﻠﻜﺮد ﮔﻴﺎه ﻋﺪس ﮔﺮدﻳﺪ. رﻗﻢ زﻳﺒﺎ در ﺳﻄﻮح ﺑﺎﻻﺗﺮ ﺳﺎﻳﻪ در ﻣﻘﺎﻳﺴﻪ ﺑﺎ رﻗﻢ ill4400 از ﻋﻤﻠﻜﺮد داﻧﻪ ﺑﻴﺸﺘﺮی ﺑﺮﺧﻮردار ﺑﻮد. اﺛﺮ ﺑﺮﻫﻤﻜﻨﺶ ﺳﺎﻳﻪ و ﺑﻴﻮﭘﺮاﻳﻤﻴﻨﮓ و رﻗﻢ ﻧﺸﺎن داد ﻛﻪ ﺑﺎ ﻋﺪم ﺳﺎﻳﻪاﻧﺪازی ﺷﺎﻫﺪ( و اﻧﺘﺨﺎب رﻗﻢ زﻳﺒﺎ و ﺗﻠﻘﻴﺢ ﺑﺎ ﺑﺎﻛﺘﺮی آزوﺳﭙﻴﺮﻳﻠﻴﻮم ﻣﻲ ﺗﻮان ﺑﻪ ﻋﻤﻠﻜﺮد داﻧﻪ ﻗﺎﺑﻞ ﻗﺒﻮﻟﻲ دﺳﺖ ﻳﺎﻓﺖ.

Study of root structure and leaf of lentils (Lens culinaris Medic) under light stress and Azospirillium bacteria

in order to study the effects of different light intensities and bio priming an experiment was conducted in two lentils cultivars to introduce the best cultivar. Experiment was evaluated using factorial arrangement based on randomized complete block design with three replications at the Agricultural Research Station of Ilam University during 2012 2013 cropping season. The treatments consisted of different levels of shading (no shading, 25, 50, 75 and 100% shading), Bio priming (inoculation with Azospirillium brasilense and without inoculation) and lentil cultivars (Ziba and ILL4400), respectively. The results indicated that shading reduced leaf specific leaf, Water saturate deficit, total number of active nodes, root fresh weight, root dry weight, root volume, main root length and grain yield, but increased leaf area index, total water content, specific leaf area, respectively. But Azospirillium leaded to reduced losses caused by shading and increased plant growth and yield of lentil. Two lentil cultivars along with inoculation with Azospirillum had the more amount of grain yield. The Ziba cultivar compared to ILL4400 cultivar had the more grain yield in the higher levels of shading. interaction effect of shading× bio priming × cultivar showed that the lack of shading (control treatment) and selection of the Ziba cultivar and inoculation with Azospirillum may achieve the acceptable grain yield, but in the conditions of intercropping and agroforestry systems that light is a limiting factor, grown Ziba cultivar along with inoculation with Azospirillum will be more successful than the ILL4400.