targetome analysis revealed involvement of mir-126 in neurotrophin signaling pathway: a possible role in prevention of glioma development

Objective: for the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of mir-126 and irs-1 in neurotrophin pathway. materials and methods: in this prospective study, validated and predicted targets (targetome) of mir-126 were collected following searching mirtarbase (http://mirtarbase.mbc.nctu.edu.tw/) and mirwalk 2.0 databases, respectively. then, approximate expression of mir-126 targeting in glioma tissue was examined using unigene database (http://www.ncbi. nlm.nih.gov/unigene). in silico molecular pathway enrichment analysis was carried out by david 6.7 database (http://david. abcc.ncifcrf.gov/) to explore which signaling pathway is related to mir-126 targeting and how mir-126 attributes to glioma development. results: mir-126 exerts a variety of functions in cancer pathogenesis via suppression of expression of target gene including pi3k, kras, egfl7, irs-1 and vegf. our bioinformatic studies implementing david database, showed the involvement of mir-126 target genes in several signaling pathways including cancer pathogenesis, neurotrophin functions, glioma formation, insulin function, focal adhesion production, chemokine synthesis and secretion and regulation of the actin cytoskeleton. conclusion: taken together, we concluded that mir-126 enhances the formation of glioma cancer stem cell probably via down regulation of irs-1 in neurotrophin signaling pathway.