association of anril expression with coronary artery disease in type 2 diabetic patients

Objective: anril is an important antisense noncoding rna gene in the ink4 locus (9p21.3), a hot spot region associated with multiple disorders including coronary artery disease (cad), type 2 diabetes mellitus (t2dm) and many different types of cancer. it has been shown that its expression is dysregulated in a variety of immune-mediated diseases. cad is a major problem in t2dm patients and the cause of almost 60% of deaths in these patients worldwide. the aim of the present study was to compare the expression level of anril between t2dm patients with and without cad. materials and methods: in this case-control study, we examined anril expression in peripheral blood mononuclear cell samples by quantitative reverse transcription- polymerase chain reaction (rt-qpcr) in 64 t2dm patients with and without cad (33 cad+ and 31 cad- patients respectively, established by coronary angiography). results: expression analysis revealed that anril was up regulated (2.34-fold, p=0.012) in cad+ versus caddiabetic patients. data from receiver operating characteristic (roc) curve analysis has shown that anril could act as a potential biomarker for detecting cad in diabetic patients. conclusion: the expression level of anril is associated with presence of cad in diabetic patients and could be considered as a potential peripheral biomarker.