semaphorin-3a as an immune modulator is suppressed by microrna-145-5p

Objective: semaphorin-3a (sema3a) and its receptors are found on some immune cells and act as suppressors of immune cells over-activation. considering the role of sema3a and its down-regulation in some autoimmune diseases, as well as our bioinformatics predictions, we assumed that mir-145-5p might affect sema3a expression. so, we aimed to determine the effect of mir-145-5p on sema3a gene expression level. materials and methods: in this experimental study, we evaluated the effect of mir-145-5p transfection on sema3a expression in peripheral blood mononuclear cells (pbmcs) using elisa and quantitative real-time polymerase chain reaction (pcr) methods. results: our results showed that mir-145-5p is able to decrease sema3a expression at both protein and mrna levels. these data confirmed our previous bioinformatic prediction about the inhibitory effect of mir-145-5p on sema3a expression. conclusion: these results enlightened us about an unknown aspect of sema3a role in some autoimmune disorders like multiple sclerosis (ms) and rheumatoid arthritis (ra) and also proposed sema3a as a potential therapeutic approach.