expression of hsa-mir-204, runx2, pparγ, and bcl2 in bone marrow derived mesenchymal stem cells from multiple myeloma patients and normal individuals

Objective: multiple myeloma (mm) is a heterogeneous cytogenetic disorder in which clonal plasma cells proliferate in the bone marrow (bm) and cause bone destruction. the bm microenvironment plays a crucial role in pathogenesis of this disease, and mesenchymal stem cells (mscs) are one of the key players. herein, we propose to investigate the expressions of hsa-mir-204, runt-related transcription factor 2 (runx2), peroxisome proliferator-activated receptor gamma (pparγ), and b-cell lymphoma 2 (bcl2) as factors involved in osteogenesis, adipogenesis, and msc survival in bm-mscs from mm patients and normal individuals. materials and methods: in this experimental study, we isolated mscs from bm aspirates of mm patients and healthy donors. total rna were extracted before and after co-culture with l363 myeloma cells. gene expressions of runx2, pparγ, bcl2, and hsa-mir-204 were assessed by quantitive real time polymerase chain reaction (qrt-pcr). results: higher levels of runx2, pparγ, and hsa-mir-204 expressions existed in mm-mscs compared to normally derived (nd)-mscs. bcl2 expression decreased in mm-mscs. we observed different results in the co-culture model. conclusion: in general, the mm-mscs gene expression profile differed compared to nd-mscs. upregulation of runx2, pparγ, and hsa-mir-204 in mm-mscs compared to nd-mscs would result in formation of bone defects. downregulation of bcl2 would lead to mm-msc cell death.