methoxyamine enhances 5-fluorouracil-induced radiosensitization in colon cancer cell line ht29

Objective: this study intended to observe the effects of methoxyamine (mx) on cytotoxic effects and dna damage caused by 5-fluorouracil (5-fu) in combination with gamma radiation in a human colon cancer cell line, ht29. materials and methods: in this experimental study, ht29 cells were cultured as a monolayer and treated with different concentrations of 5-fu along with 1 mm mx for 24 hours. next, the cells were irradiated with 2 gy gamma radiation. after the treatments, we assessed for dna damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays. results: cytotoxicity and dna damage increased with increasing 5-fu concentration. the 1 mm mx concentration had no significant effect on cytotoxicity and dna damage from 5-fu; however, it increased the cytotoxic and genotoxic effects of different concentrations of 5-fu when used in combination with 2 gy gamma radiation. conclusion: mx combined with 5-fu enhanced the radiosensitivity of colon cancer cells.