Experimental Model of Pulmonary Allergic Inflammation Induced By Latex in Balb/C

Introduction: the prevalence of allergic airway diseases has dramaticallyincreased in recent years all over the world. murine models of allergic airwayinflammation have provided helpful information about treatment and cellularand molecular mechanisms of the disease. previous published works usingmurine models to investigate latex allergy did not introduce a completecharacteristic eosinophilic allergic airway inflammation. latex allergy isimportant due to serious health impacts and widespread use of its products.thus, the aim of this study was to establish a new mouse model of latexallergic airway inflammation using aerosol inhalation.material and methods: initially, four groups of mice were injectedintraperitoneally (ip) with 0, 10, 50, or 200 ~g of latex extract and their serumanti-latex ige titers were compared using elisa to find out the optimumdose for ip injection.in the second stage, a standard protocol of inhalation was designed andthree doses of latex extract solutions including 1%, 0.1%, and 0.01% wereused to induce allergic airway inflammation. characteristics of this modelwere shown by studying different parameters including bronchoalveolarlavage (bal), cytokines (interleukin-5 [il-5] and interleukin-13 [il-13]) andserum anti-latex ige and igg1 titers by elisa, specific histologic changes inthe lung and eosinophilia of the bone marrow, peripheral blood, bal, andlung inflammatory foci.results: the aerosol inhalation of 1% latex allergens solution and presensitizationwith 50 ~g of latex in this study resulted in the development ofcharacteristic allergic airway inflammation in balb/c mice. these featuresincluded elevated allergen specific ige and igg1, peripheral blood, bal andbone marrow eosinophilia and characteristic inflammatory response in lungwith eosinophil infiltration. elevated levels of il-5 and il-13 can be a sign ofthis type of inflammation.conclusion: this paper describes a latex aerosol inhalational challengemodel of eosinophilic airway inflammation in latex pre-sensitized balb/cmice.