Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells

Objective: micro-rnas (mirnas) are a class of posttranscriptional regulators that play crucial roles in various biological processes. emerging evidence suggests a direct link between mirnas and development of several diseases including type 2 diabetes (t2d). in this study, we aimed to investigate the effect of predicted mirna and target genes on insulin resistance. materials and methods: this experimental study was conducted on the c2c12 cell line. using bioinformatics tools mirna-135 and two respective target genes-insulin receptor (insr) and vesicle associated membrane protein 2 (vamp2)- were selected as potential factors involved in insulin resistance process. levels of glucose uptake mirna expression and respective gene targets were determined after cell transfaction by mir-135. results: it was determined that insr gene expression was significantly down-regulated in mir-135 transfected c2c12 cell line (p≤0.05). interestingly; these transfected cells have shown a significant difference in glucose uptake incomparision the positive control cells, while it was similar to the insulin resistant cell line (p≤0.05). in contrast, no significant alteration of vamp2 gene expression was observed. conclusion: our data indicated no change on the vamp2 expression level after mirna transfection, while expression level of insr was reduced and mir-135 expression was contrarily increased leading to poor stimulation of glucose uptake through insulin, and development of insulin resistance phenotype in c2c12 cell line.