Efficacy of Hyaluronic Acid in the Selection of Human Spermatozoa with Intact DNA by the Swim-up Method

Objective: in 2014, enrolled 20 patients who applied to the unit of assisted reproduction techniques, konya necmettin erbakan university. based on the presence of hyaluronic acid (ha) in the oocyte-cumulus cell complex, sperm attached to ha in vivo were modeled in vitro. available healthy sperm obtained in the swim-up procedure using ha were investigated. materials and methods: this observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. we divided each sample into two groups and the swim-up method was applied. human serum albumin (hsa, 0.5%) was added to samples from the first group. ha (10%) was added to samples from the second group. we determined the floating linear and non-linear sperm concentrations of both groups annexin v was used to determine the rate of apoptosis of these sperm. results: following swim-up, linear and non-linear sperm concentrations were higher in the group that contained ha compared to the group with hsa. however, there was a significantly higher apoptosis rate in the hsa group compared to the ha group. conclusion: the addition of ha to the medium in the swim-up procedure positively affected sperm parameters. thus, healthier sperm cells were obtained without dna damage and with high motility.