Determination of Asymptomatic Chlamydia Trachomatis Infections By Omp1 Gene Based -Per

The objective of this research was to determine the prevalenceof genital c. trachomatis infection in asymptomatic women by using highlysensitive nested-polymerase chain reaction (pcr) in urine sample.materials and methods: one hundred-forty asymptomatic women wererandomly selected from those who attended gynecology out patient departmentof hazraat e rasool hospital intehran. first catch urine specimen were collectedfrom all the participants . dna extraction was performed by means of highpure pcr template preparation kit (hpptp) according to the manufacture'sinstructions. extracted dna was tested by omp1 gene based nested-pcr,using sets of primers to amplify c. trachomatis omp1 gene. visualizationof a 1027 bp fragment from omp1 gene in agarose gel electrophoresis wasconsidered as a positive result.results: in total , 140 urines were tested for determination of c. trachomatisinfection. c. trachomatis omp-1 was detected in 22.1% of cases (31/140). theoverall prevalence rates of c. trachomatis in the urine sample as determined byomp1 based nested-ptlr were 4.3% in group i (age, <25 years), 12.1% in groupii (age, 25-34 years), 5.0% in group iii (age, 35-44 years) and 0.7% in group iv(>44 years). the highest prevalence of c. trachomatis infection (12.1%) wasseen in women aged 25-34 years. this finding was not statistically significant(p=o.710). also, there was not relation between c. trachomatis infection andsome probable risk factors such as young age «25 years), sto history andmissing use of barrier contraceptive in this study.conclusion: the prevalence of c.trachomatis infection in the women notseeking health care warrants more comprehensive study using high sensitiveomp1 based nested- pcr to identify and treat a large number of infectedwomen in iran.