Restoration of CpG Methylation in the Egf Promoter Region during Rat Liver Regeneration

Epidermal growth factor (egf) is an important factor for healing after tissue damage indiverse experimental models. it plays an important role in liver regeneration (lr). theobjective of this experiment is to investigate the methylation variation of 10 cpg sites inthe egf promoter region and their relevance to egf expression during rat liver regeneration.as a follow up of our previous study, rat liver tissue was collected after rat 2/3 partialhepatectomy (ph) during the re-organization phase (from days 14 to days 28). liver dnawas extracted and modified by sodium bisulfate. the methylation status of 10 cpg sites inegf promoter region was determined using bisulfite sequencing polymerase chain reaction(pcr), as bsp method. the results showed that 3 (sites 3, 4 and 9) out of 10 cpg siteshave strikingly methylation changes during the re-organization phase compared to theregeneration phase (from 2 hours to 168 hours, p=0.002, 0.048 and 0.018, respectively).our results showed that methylation modification of cpgs in the egf promoter region couldbe restored to the status before ph operation and changes of methylation didn’t affect egfmrna expression during the re-organization phase.