Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite

Objective: in this study, nano-biocomposite composed of poly (lactide-co-glycolide)(plga) and chitosan (cs) were electrospun through a single nozzle by dispersing the csnano-powders in plga solution. the cellular behavior of human adipose derived stemcells (h-adscs) on random and aligned scaffolds was then evaluated.materials and methods: in this experimental study, the plga/cs scaffolds were preparedat the different ratios of 90/10, 80/20, and 70/30 (w/w) %. morphology, cell adhesion and proliferationrate of h-adscs on the scaffolds were assessed using scanning electron microscope(sem), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (mtt) assay and trypanblue staining respectively.results: h-adscs seeded on the matrices indicated that the plga/cs composite matrixwith aligned nanofibres and higher content of cs nano-powders gave significantly betterperformance than others in terms of cell adhesion and proliferation rate (p < 0.05).conclusion: we found that cs enhanced cell adhesion and proliferation rate, andaligned nanofibers guided cell growth along the longitudinal axis of the nanofibers,which would provide a beneficial approach for tissue engineering.