Expression Change of miR-214 and miR-135 during Muscle Differentiation

Objective: micrornas (mirnas) are a class of small non-coding rnas that play pivotalroles in many biological processes such as regulating skeletal muscle development wherealterations in mirna expression are reported during myogenesis. in this study, we aimedto investigate the impact of predicted mirnas and their target genes on the myoblast tomyocyte differentiation process.materials and methods: this experimental study was conducted on the c2c12 cell line.using a bioinformatics approach, mir-214 and mir-135 were selected according to theirtargets as potential factors in myoblast to myocyte differentiation induced by 3% horseserum. immunocytochemistry (icc) was undertaken to confirm the differentiation processand quantitative real-time polymerase chain reaction (pcr) to determine the expressionlevel of mirnas and their targets.results: during myoblast to myocyte differentiation, mir-214 was significantly downregulatedwhile mirna-135, irs2, akt2 and insr were overexpressed during the process.conclusion: mir-214 and mir-135 are potential regulators of myogenesis and areinvolved in skeletal muscle development through regulating the irs/pi3k pathway.