Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells With Platelet-Rich Plasma Accelerate Distraction Osteogenesis in A Canine Model

Objective: distraction osteogenesis (do) is a surgical procedure used to generate largevolumes of new bone for limb lengthening.materials and methods: in this animal experimental study, a 30% lengthening of the lefttibia (mean distraction distance: 60.8 mm) was performed in ten adult male dogs by callusdistraction after osteotomy and application of an ilizarov fixator. distraction was started onpostoperative day seven with a distraction rate of 0.5 mm twice per day and carried out ata rate of 1.5 mm per day until the end of the study. autologous bone marrow mesenchymalstem cells (bm-mscs) and platelet-rich plasma (prp) as the treatment group (n=5)or prp alone (control group, n=5) were injected into the distracted callus at the middleand end of the distraction period. at the end of the consolidation period, the dogs weresacrificed after which computerized tomography (ct) and histomorphometric evaluationswere performed.results: radiographic evaluationsrevealed that the amount and quality of callus formationswere significantly higher in the treatment group (p < 0.05). as measured byct scan, the healing parametersin dogs of the treatment group were significantlygreater (p < 0.05). new bone formation in the treatment group was significantly higher(p < 0.05).conclusion: the present study showed that the transplantation of bm-mscs positivelyaffects early bony consolidation in do. the use of mscs might allow a shortened periodof consolidation and therefore permit earlier device removal.