Effect of Superparamagnetic Iron Oxide Nanoparticles-Labeling on Mouse Embryonic Stem Cells

Objective: superparamagnetic iron oxide nanoparticles (spions) have been used to labelmammalian cells and to monitor their fate in vivo using magnetic resonance imaging(mri). however, the effectiveness of phenotype of labeled cells by spions is still a matterof question. the aim of this study was to investigate the efficiency and biological effectsof labeled mouse embryonic stem cells (mescs) using ferumoxide- protamine sulfatecomplex.materials and methods: in an experimental study, undifferentiated mescs, c571 line, agenerous gift of stem cell technology company, were cultured on gelatin-coated flasks.the proliferation and viability of spion-labeled cells were compared with control. escsand embryoid bodies (ebs) derived from differentiated hematopoietic stem cells (hscs)were analyzed for stage-specific cell surface markers using fluorescence-activated cellsorting (facs).results: our observations showed that spions have no effect on the self-renewal abilityof mescs. reverse microscopic observations and prussian blue staining revealed 100%of cells were labeled with iron particles. spion-labeled mescs did not significantly altercell viability and proliferation activity. furthermore, labeling did not alter expression ofrepresentative surface phenotypic markers such as stage-specific embryonic antigen 1(ssea1) and cluster of differentiation 117 (cd117) on undifferentiated esc and cd34,cd38 on hscs, as measured by flowcytometry.conclusion: according to the results of the present study, spions-labeling methodas mri agents in mescs has no negative effects on growth, morphology, viability,proliferation and differentiation that can be monitored in vivo, noninvasively. noninvasivecell tracking methods are considered as new perspectives in cell therapy forclinical use and as an easy method for evaluating the placement of stem cells aftertransplantation.