Treatment of Hypertrophic Scar in Human with Autologous Transplantation of Cultured Keratinocytes and Fibroblasts along with Fibrin Glue

Objective: hypertrophic scar involves excessive amounts of collagen in dermal layer andmay be painful. nowadays, we can’t be sure about effectiveness of procedure for hypertrophicscar management. the application of stem cells with natural scaffold has been thebest option for treatment of burn wounds and skin defect, in recent decades. fibrin glue(fg) was among the first of the natural biomaterials applied to enhance skin deformity inburn patients. this study aimed to identify an efficient, minimally invasive and economicaltransplantation procedure using novel fg from human cord blood for treatment of hypertrophicscar and regulation collagen synthesis.materials and methods: in this case series study, eight patients were selected with hypertrophicscar due to full-thickness burns. human keratinocytes and fibroblasts derivedfrom adult skin donors were isolated and cultured. they were tested for the expression ofcytokeratin 14 and vimentin using immunocytochemistry. fg was prepared from pooledcord blood. hypertrophic scars were extensively excised then grafted by simply placingthe sheet of fg containing autologous fibroblast and keratinocytes. histological analyseswere performed using hematoxylin and eosin (h&e) and masson’s trichrome (mt) stainingof the biopsies after 8 weeks.results: cultured keratinocytes showed a high level of cytokeratin 14 expression andalso fibroblasts showed a high level of vimentin. histological analyses of skin biopsiesafter 8 weeks of transplantation revealed re-epithelialization with reduction of hypertrophicscars in 2 patients.conclusion: these results suggest may be the use of fg from cord blood, which is notmore efficient than previous biological transporters and increasing hypertrophic scarrelapse, but could lead to decrease pain rate.