Association Study Between Coronary Artery Disease and Rs1333049 and Rs10757274 Polymorphisms At 9p21 Locus in South-West Iran

Objective: coronary artery disease (cad) is a multi-factorial and heterogenic diseasewith atherosclerosis plaques formation in internal wall of coronary artery. plaque formationresults to limitation of the blood reaching to myocardium leading to appearanceof some problems, such as ischemia, sudden thrombosis veins and myocardialinfarction (mi). several environmental and genetic factors are involved in prevalenceand incident of cad as follows: hypertension, high low density lipoprotein-cholesterol(ldl-c), age, diabetes mellitus, family history of early-onset heart disease and smoking.according to genome wide association studies (gwas), five polymorphisms in the9p21 locus seem to be associated with the cad. we aimed to evaluate the remarkableassociation of two polymorphisms at 9p21 locus, rs1333049 and rs10757274,with cad.materials and methods: this experimental study was conducted in golestan, aria hospitalsand genetics lab of shahid chamran university in the city of ahvaz, iran, in 2010-2011. the collected blood samples belonging to 170 cad patients (case group) and 100healthy individuals (control group) were analyzed by tetra-primer amplification refractorymutation system (arms)-polymerase chain reaction (pcr) technique. the results wereanalyzed using software package used for statistical analysis (spss; spss inc., usa)version 16. a value of p < 0.05 and an odd ratio (or) with 95% confidence intervals (ci)were considered significant.results: the frequencies of cc, cg and gg genotypes for rs1333049 polymorphismin patients were 18.2, 65.3 and 16.5%, while in controls, the related values were 25,67 and 8%, respectively. gg genotypes of rs1333049 polymorphism in cad patientswere more than control cases (or: 0.354, 95%ci: 0.138-0.912, p=0.032). the frequenciesof aa, ag and gg genotypes for rs10757274 in cad patients were 8.2, 58.3and 33.5%, while in controls, the related values were 35, 63 and 2%, respectively. gggenotype in rs10757274 polymorphism in cad patients was found more than controlcases (or: 0.014, 95% ci: 0.003 -0.065, p=0.0001).conclusion: the rs1333049 polymorphism at 9p21 locus shows a weak association withcad, whereas rs10757274 polymorphism reveals a significant association with cad.these variants may help the identification of patients with increased risk for coronaryartery disease.