Morphological Study of Isolated Ovarian Preantral Follicles Using Fibrin Gel Plus Platelet Lysate after Subcutaneous Transplantation

Objective: ovarian and follicle transplantation may preserve fertility in young cancer survivors.in this study, we have transplanted preantral follicles using fibrin gel as a carrierand fibrin gel supplemented with platelet lysate (pl) as a rich source of angiogenic andgrowth factors. the purpose of this study was to evaluate the role of fibrin gel and pl infollicle transplantation.materials and methods: in this experimental study, ovaries were taken from 14-dayoldnaval medical research institute (nmri) mice. preantral follicles were dissectedfrom the ovaries and encapsulated into fibrin gel supplemented with 5, 10, 15 or 20%pl, then transplanted back into the same donor mice. fibrin gels supplemented withpl that contained preantral follicles were placed in a subcutaneous pocket in theback of the neck of the recipient, donor mouse (the same mouse that follicles werecollected). after 14 days the grafts were processed and embedded in paraffin blocks,then serially sectioned for histological evaluation. we counted the follicles and classifiedthem according to stage (preantral or antral). data were presented as mean ±standard error of mean (sem) and analysed by analysis of variance (anova) and thekruskal-wallistest.results: the mean percentage of recovered follicles encapsulated and transplantedin each group were 33.30 ± 2.47 (fibrin gel), 31.96 ± 1.90 (fibrin gel+5% pl), 34.02± 2.44 (fibrin gel+10% pl), 48.31 ± 2.06 (fibrin gel+15% pl) and 17.60 ± 2.79 (fibringel+20% pl). there was a signi?cant increase in the recovery rate of grafted follicleswith fibrin gel+15% pl (48.31%; p < 0.001). the percentage of preantral folliclesshowed no significant difference in all groups (p < 0.05). the percentage of antral folliclesshowed a significant decrease in follicles grafted with fibrin gel+20% pl whencompared to the other groups (11.77%; p < 0.005) but no significant difference wasobserved in the other groups.conclusion: the use of pl in follicle transplantation can improve ovarian follicularsurvival rate.