Evaluation of Mrna Contents of Ybx2 and Jhdm2a Genes on Testicular Tissues of Azoospermic Men With Different Classes of Spermatogenesis

Objective: animal model studies have shown that msy2 and jhdm2a genes have animportant role in spermatogenesis process and fertility of male mice. but the potentialrole of these genes in human spermatogenesis and fertility is not known yet. therefore,we evaluated expression ratios of these genes in testis tissues of men with normal andimpaired spermatogenesis.materials and methods: in this experimental study, after rna extraction and cdna synthesisfrom 50 non-obstructive azoospermic and 12 normal testis tissues, the expressionratios of genes were evaluated by real time polymerase chain reaction (pcr) technique.hematoxcylin and eosin (h&e) staining was used for histological classification of testis tissues.for statistical analysis, one way analysis of variance (anova) test was carried out.results: our results showed a significant reduction in mrna level of ybx2 in sampleswith impaired spermatogenesis (p < 0.001) compared to samples with qualitatively normalspermatogenesis and normal spermatogenesis; however, in jhdm2a gene, despitesensible reduction in gene expression level in men with impaired spermatogenesis, nosignificant differences were shown (p > 0.05). furthermore in ybx2, a significant negativecorrelation was demonstrated between the efficiency score of spermatogenesis and thethreshold cycle (ct) (r=-0.7, p < 0.0001), whereas in jhdm2a, this negative correlationwas not significant (r=-0.4, p=0.06).conclusion: generally, these data indicated that ybx2 and jhdm2a genes may playan important role in male infertility, and suggested that these molecules can act asuseful biomarkers for predicting male infertility.