Reliability Evaluation of Fluorescence in Situ Hybridization (FISH) and G-Banding on Bone Marrow and Peripheral Blood Cells in Chronic Myelogenous Leukemia Patients

Chronic myeloid leukemia (cml) is a myeloproliferative disease. the cytogenetic hallmarkof cml is philadelphia (ph) chromosome. this study aimed to diagnose suspected cmlpatients, to monitor cml patients under therapy using cytogenetic and fluorescence insitu hybridization (fish) techniques to analyze their bone marrow (bm) and peripheralblood (pb) samples, and finally to compare their obtained results for both specimens. thisstudy was conducted during one-year period (2012-2013). the participants were recruitedfrom the hematology and oncology clinic of shahid gazi (emam reza) hospital of tabrizuniversity of medical sciences, tabriz, east azerbaijan province, iran. we analyzed 90samples from 60 suspected cml patients (30 bm and 60 pb samples). all samples wereanalyzed using g-banding, 5 samples using dual fusion fish (df-fish) probes, as wellas 30 samples using both fish and g-banding. among the 90 analyzed samples of 60 patients,25 (41.66%) were ph+ using karyotyping, whereas five cases were not analyzable,so fish was applied and the results confirmed that only two individuals were bcr-abl+.in the comparison between 25 bm and 25 pb samples using karyotyping, 15 (60%) and10 (40%) were ph+, respectively. the comparison of fish and karyotyping on 30 samplesshowed that 9 (30%) and 8 (26.66%) were ph+, respectively, and only 18.18% of ph+patients showed atypical patterns. in the comparison between bm-cytogenetic and pbinterphase-fish (i-fish), bm-cytogenetic was more reliable than pb-i-fish in detectingph. our data demonstrate that fish analysis is a rapid, reliable and sensitive technique.the comparison between bm and pb showed that pb can not be replaced by bm, evenin detecting by fish.