The Effect of Baicalin As A Ppar Activator on Erythroid Differentiation of Cd133+ Hematopoietic Stem Cells in Umbilical Cord Blood

Objective: the peroxisome proliferator-activated receptors (ppars) are a group of nuclearreceptor proteins whose functions as transcription factors regulate gene expressions.ppars play essential roles in the regulation of cellular differentiation, development,and metabolism (carbohydrate, lipid, protein), and tumorigenesis of higher organisms.this study attempts to determine the effect of baicalin, a ppar? activator, on erythroiddifferentiation of cluster of differentiation 133+ (cd133+) cord blood hematopoietic stemcells (hscs).materials and methods: in this experimental study, in order to investigate the effects ofthe ppar? agonists baicalin and troglitazone on erythropoiesis, we isolated cd133+ cellsfrom human umbilical cord blood using the macs method. isolated cells were culturedin erythroid-inducing medium with or without various amounts of the two ppar? activators(baicalin and troglitazone). erythroid differentiation of cd133+ cord blood hscs wereassessed using microscopic morphology analysis, flow cytometric analysis of erythroidsurface markers transferrin receptor (tfr) and glycophorin a (gpa) and bycolony formingassay.results: microscopic and flow cytometric analysis revealed the erythroid differentiation ofcd133+ cord blood hscs under applied erythroid inducing conditions. our flow cytometricdata showed that the tfr and gpa positive cell population diminished significantly in thepresence of either troglitazone or baicalin. the suppression of erythroid differentiationin response to ppar? agonists was dose-dependent. erythroid colony-forming ability ofhsc decreased significantly after treatment with both ppar? agonists but troglitazonehad a markedly greater effect.conclusion: our results have demonstrated that ppar? agonists modulate erythroid differentiationof cd133+ hscs, and therefore play an important role in regulation of normalerythropoiesis under physiologic conditions. thus, considering the availability and applicationof this herbal remedy for treatment of a wide range of diseases, the inhibitory effect ofbaicalin on erythropoiesis should be noted.