A Comparative Study of Osteogenic Differentiation Human Induced Pluripotent Stem Cells and Adipose Tissue Derived Mesenchymal Stem Cells

Objective: human induced pluripotent stem cells (ipscs) have been shown to havepromising capacity for stem cell therapy and tissue engineering applications. therefore,it is essential to compare the ability of these cells with the commonly used mesenchymalstem cells (msc) for bone tissue engineering in vitro.materials and methods: in this experimental study, the biological behavior and osteogeniccapacity of the ipscs were compared with mscs isolated from human adipose tissue(at-mscs) using 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt)assay, alizarin red staining, alkaline phosphatase (alp) activity measurements, calciumcontent assay and common osteogenic-related genes. data were reported as the mean ±sd. one-way analysis of variance (anova) was used to compare the results. a p value ofless than 0.05 was considered statistically significant.results: there was a significant difference between the rate of proliferation of the twotypes of stem cells; ipscs showed increased proliferation compared to at-mscs. duringosteogenic differentiation, alp activity and mineralization were demonstrated to besignificantly higher in ipscs. although at-mscs expressed higher levels of runx2, ipscsexpressed higher levels of osteonection and osteocalcin during differentiation.conclusion: ipscs showed a higher capacity for osteogenic differentiation and hold promisingpotential for bone tissue engineering and cell therapy applications.