The Effects of Plasma Treated Electrospun Nanofibrous Poly (ε-caprolactone) Scaffolds with Different Orientations on Mouse Embryonic Stem Cell Proliferation

Objective: assessments of cell reactions such as motility, orientation and activation tothe topography of the substratum will assist with the fabrication of a proper implantablescaffold for future tissue engineering applications.the current challenge is to analyze theorientation effect of elecrospun nanofibers of poly (?-caprolactone) (pcl) on viability andproliferation of mouse embryonic stem cells (mescs).materials and methods: in this experimental study, we used the electrospinning methodto fabricate nanofibrous pcl scaffolds. chemical and mechanical characterizations werespecified by the contact angle and tensile test. o2 plasma treatment was used to improvesurface hydrophilicity. we used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (mtt) assay to evaluate mescs adhesion and proliferation before and after surfacemodification. the influence of the orientation of the nanofibers on mescs growth wasevaluated by scanning electron microscopy (sem). statistical analysis was performedusing one-way analysis of variance (anova) with differences considered statistically significantat p?0.05.results: the results showed that plasma treatment improved the hydrophilic propertyof pcl scaffolds. mtt assay showed a significant increase in proliferation of mescs onplasma treated pcl (p-pcl) scaffolds compared to non-treated pcl (p?0.05). howevergelatin coated tissue culture plate (tcp) had a better effect in initial cell attachment afterone day of cell seeding. there was more cell proliferation on day 3 in aligned plasmatreated (ap) nanofibers compared to the tcp. sem showed optical density of the cellcolonies. aligned nanofibrous scaffolds had larger colony sizes and spread more thanrandom nanofibrous scaffolds.conclusion: this study showed that plasma treating of scaffolds was a more suitablesubstrate for growth and cell attachment. in addition, aligned nanofibrous scaffolds highlysupported the proliferation and spreading of mescs when compared to random nanofibrousscaffolds and tcp.