The Comparison of the Effects of Silybin and Silybin-Phosphatidylcholine on Viability and Esr Expression in Human Breast Cancer T47d Cell Line

Objective: silybin is a polyphenol with anti-oxidant and anti-cancer properties. the poorbioavailability of some polyphenols can be improved by binding to phosphatidylcholine. inrecent years, studies have been conducted to evaluate the anti-cancer effect of silybin.we studied the effect of silybin and silybin-phosphatidylcholine on esr1 and esr2 geneexpression and viability in the t47d breast cancer cell line.materials and methods: in this experimental study, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (mtt test) was used to determine doses for cell treatment,and the gene expression was analyzed by real-time reverse transcriptase-polymerasechain reaction (real-time rt- pcr).results: significant dose- and time-dependent cell growth inhibitory effects of silybin andsilybin-phosphatidylcholine along with esr1 down-regulation were observed in t47dcells. in contrast to esr1, the t47d cell line showed negligible esr2 expression.conclusion: this study suggests that silybin and silybin-phosphatidylcholine down-regulateesr1 in er+ breast cancers. results also show that in the t47d cell line, silybinphosphatidylcholinehas a much higher growth inhibitory effect and a more significantdown-regulation of esr1 compared with silybin.