A Rare De novo Complex Chromosomal Rearrangement (CCR) Involving Four Chromosomes in An Oligo-asthenosperm Infertile Man

Complex chromosomal rearrangements (ccrs) are rare events involving more thantwo chromosomes and over two breakpoints. they are usually associated with infertilityor sub fertility in male carriers. here we report a novel case of a ccr in a30-year-old oligoasthenosperm man with a history of varicocelectomy, normal testessize and normal endocrinology profile referred for chromosome analysis to thegenetics unit of royan reproductive biomedicine research center. chromosomalanalysis was performed using peripheral blood lymphocyte cultures and analyzedby gtg banding. additional tests such as c-banding and multicolor fluorescence insitu hybridization (fish) procedure for each of the involved chromosomes were performedto determine the patterns of the segregations. y chromosome microdeletionsin the azoospermia factor (azf) region were analyzed with multiplex polymerasechain reaction. to identify the history and origin of this ccr, all the family memberswere analyzed. no micro deletion in y chromosome was detected. the same de novoreciprocal exchange was also found in his monozygous twin brother. the other siblingsand parents were normal. ccrs are associated with male infertility as a resultof spermatogenic disruption due to complex meiotic configurations and the productionof chromosomally abnormal sperms. these chromosomal rearrangements mighthave an influence on decreasing the number of sperms.