Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells

Objective: there is constant difficulty in obtaining adequate supplies of blood components,as well as disappointing performance of universal red blood cells. advances insomatic cell reprogramming of human-induced pluripotent stem cells (hipscs) have provideda valuable alternative source to differentiate into any desired cell type as a therapeuticpromise to cure many human disease.materials and methods: in this experimental study, we examined the erythroid differentiationpotential of normal bombay hipscs (b-hipscs) and compared resultsto human embryonic stem cell (hesc) lines. because of lacking abo blood groupexpression in b-hipscs, it has been highlighted as a valuable source to produce anycell type in vitro.results: similar to hesc lines, hemangioblasts derived from b-hipscs expressed approximately9% kdr+cd31+ and approximately 5% cd31+cd34+. in semisolid media,ipsc and hesc-derived hemangioblast formed mixed type of hematopoietic colony. inmixed colonies, erythroid progenitors were capable to express cd71+gpa+hbf+ and accompaniedby endothelial cells differentiation.conclusion: finally, ips and es cells have been directly induced to erythropoiesis withouthemangioblast formation that produced cd71+hbf+erythroid cells. although we observedsome variations in the efficiency of hematopoietic differentiation between ipsc and es cells,the pattern of differentiation was similar among all three tested lines.