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The Effects of Exendine-4 on Insulin Producing Cell Differentiation From Rat Bone Marrow-Derived Mesenchymal Stem Cells
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نویسنده
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Nejad-Dehbashi Fereshteh ,Hashemitabar Mahmoud ,Orazizadeh Mahmoud ,Bahramzadeh Somaieh ,Shahhosseini Pourshoushtary Elham ,Khorsandi Layasadat
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منبع
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Cell Journal (Yakhteh) - 2014 - دوره : 16 - شماره : 2 - صفحه:187 -194
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چکیده
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Objective: the aim of this study was to evaluate the effect of exendin-4 (ex-4) on differentiation of insulin-producing cells (ipcs) from rat bone marrow-derived mesenchymal stem cells (rat-bm-mscs).materials and methods: in this experimental study, rat-bm-mscs were cultured and the cells characterized by flow cytometry analysis of cell surface markers. rat-bm-mscs were subsequently treated with induction media with or without ex-4. after induction, the presence of ipcs was demonstrated with dithizone (dtz) staining and gene expression profiles for pancreatic cell differentiation markers (pdx-1, glut-2, insulin) were assessed using reverse transcription polymerase chain reaction (rt-pcr). insulin excreted from differentiated cells was analyzed with radioimmunoassay (ria). the two-tailed student’s t-test was used for comparison of the obtained values.results: the percentage of dtz-positive cells significantly increased in ex-4 treated cells (p < 0.05). expression of the islet-associated genes pdx-1, glut-2 and insulin genes in ex-4 treated cells was markedly higher than in the cells exposed to differentiation media without ex-4. ria analysis demonstrated significant release of insulin with the glucose challenge test in ex-4 treated cells compared to ex-4 untreated cells.conclusion: the results of this study have demonstrated that ex-4 can enhance differentiation of ipcs from rat-bm-mscs.
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کلیدواژه
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Exendin-4 ,Mesenchymal Stem Cells ,Insulin-Producing Cells ,Pdx-1 ,Glut-2
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آدرس
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Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران, Ahvaz Jundishapur University Of Medical Sciences, ایران
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پست الکترونیکی
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layasadat@yahoo.com
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Authors
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Elham ,Fereshteh ,Layasadat ,Mahmoud ,Mahmoud ,Somaieh
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