Expression of E-Cadherin, Leukemia Inhibitory Factor and Progesterone Receptor in Mouse Blastocysts after Ovarian Stimulation

Objective: the appropriate interaction between a blastocyst and the endometrium is essential for successful implantation. numerous factors, including hormone receptors (progesterone receptor),cytokines [leukemia inhibitory factors (lif)], and adherence molecules such as e-cadherinareinvolvedinthe cross-talkthatoccursbetween the embryoand endometrium.studiesshowthatalackofthesegenesimpactendometrialreceptivity.inthisstudy,wecomparetheexpression levels of e-cadherin, lif, and progesterone receptor (pgr) genes in blastocysts that have been obtained from superovulated mice to those obtained from natural cycles.materials and methods: in this experimental study, for the experimental group, a total of 17 virgin female nmri mice (6- 8 weeks old) were injected with 7.5 iu pregnant mare serum gonadotropin(pmsg). their blastocysts (approximately n= 120) were flushed out after 3.5 days, following administration of human chorionic gonadotropin (hcg). the control group consisted of blastocysts from 62 female mice that were mated with male mice. the natural cycle blastocysts were flushed out from the female mice uteri 3.5 days after mating. the expression levels of ecadherin,lif,tpgr geneswereexaminedbyquantitativereal-timereverse-transcriptasepolymerasechainreaction(rt-pcr).datawereanalyzedbythestudent’st-test(onesamplet-test).results: expression levels of all studied genes were significantly lower in the hormonetreatedgroup compared to the natural cycle blastocysts (p < 0.05). conclusion: although ovarian stimulation is utilized to obtain more oocytes in art cycles,it seems that this could disadvantageous toimplantation becauseof the decrease inexpression levels of certaingenes. because of theimportantroles of e-cadherin,lif,andprogesteronereceptor inthe implantation process,we have shownlowerexpressionlevelsofthese genes in mouse blastocysts obtained fromovarian-stimulated micethan thosederived from the natural cycle.theresults observed inthis study have shown thepossibilityof an unfavorable effecton implantation and pregnancy rate.